Coding

Part:BBa_K2915285

Designed by: ARRINDELL Jennifer   Group: iGEM19_Aix-Marseille   (2019-10-19)


IPTG inducible promoter (T7) with RBS TAL3 6-His-Tag with TEV site

The TAL3 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CTGTCG 3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL3, which allows the production TAL3 with its HisTag using E.coli DH5-alpha/BL21 E3 bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 639
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 252


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